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The St. Augustine Light Station is a private-aid to navigation and an active, working lighthouse in St. Augustine, Florida. The current lighthouse stands at the north end of Anastasia Island and was built between 1871 and 1874. The tower is the second lighthouse tower in St. Augustine, the first being lit officially by the American territorial government in May 1824 as Florida's first lighthouse. However, both the Spanish and the British governments operated a major aid to navigation here including a series of wooden watch towers and beacons dating from 1565.

The current lighthouse tower, original first-order Fresnel Lens and the Light Station grounds are owned by the St. Augustine Lighthouse & Maritime Museum, Inc., a not-for-profit maritime museum. The museum is open to the public 360 days a year. Admission fees support continued preservation of the lighthouse and five other historic structures. Admissions and museum memberships also fund programs in maritime archaeology, traditional wooden boatbuilding, and maritime education. The non profit mission is to "discover, preserve, present and keep alive the stories of the nation's oldest port as symbolized by our working St. Augustine Lighthouse."