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The Immaculate Collection is the first greatest hits album by American singer Madonna, released on November 9, 1990, by Sire Records. It contains new remixes of fifteen of her hit singles from 1983 to 1990, as well as two new songs. Its title is a pun on the Immaculate Conception, the conception of the Virgin Mary without the stain of original sin. An extended play titled The Holiday Collection was released in Europe to accompany the compilation and the re-release of "Holiday" which included three hits not included on the main compilation. It is the first album ever to use the audio technology QSound.

In the United States, The Immaculate Collection reached number two on the Billboard 200, while topping the album charts in the United Kingdom. It became Madonna's second album to be certified Diamond by the Recording Industry Association of America (RIAA) for shipments of over ten million copies in the United States. It also spent the second highest number of consecutive weeks at number one for a female solo artist in the United Kingdom, being at number one for a nine-week stint, before being surpassed by Adele's 21. Additionally, the album topped the charts in Australia, Canada and Finland, and the top-five in France, the Netherlands and Spain.

"Justify My Love", the album's lead single, became Madonna's ninth number-one single on the Billboard Hot 100. Its music video included sexual themes and was banned by MTV, becoming one of Madonna's most controversial singles. "Rescue Me" was released as the second single and became the highest-debuting single on the Hot 100 by a female artist at that time, entering the chart at number 15 and eventually peaking at number nine. "Holiday" was reissued in Europe as the album's third single and peaked at nine on the UK Singles Chart. The album has sold 30 million copies worldwide, making it the best-selling compilation album by a solo artist and one of the best-selling albums of all time.The Immaculate Collection would be later superseded by Madonna's third greatest hits album Celebration (2009), which includes every song from the album with the exception of "Rescue Me".