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"Trust No 1" is the sixth episode of the ninth season of the American science fiction television series The X-Files. It premiered on the Fox network on January 6, 2002. The episode was written by series creator Chris Carter and executive producer Frank Spotnitz, and directed by Tony Wharmby. "Trust No 1" helps to explore the series' overarching mythology. The episode received a Nielsen household rating of 5.1 and was viewed by 8.4 million viewers; it garnered mixed to negative reviews from television critics, with many feeling that it portrayed the series' characters in a way that was unfaithful to the show's history.

The show centers on FBI special agents who work on cases linked to the paranormal, called X-Files; this season focuses on the investigations of John Doggett (Robert Patrick), Monica Reyes (Annabeth Gish), and Dana Scully (Gillian Anderson). In this episode, Scully is hopeful about reuniting with her former partner, Fox Mulder (David Duchovny) when a complete stranger offers new information about what drove him into hiding. Yet her trust in the stranger may place Mulder in even more danger, for the man turns out to be a super soldier.

"Trust No 1" features former leading star Duchovny via the use of previously filmed footage. It was written in response to fans who felt that, during season eight, Mulder's abduction was not dealt with until his miraculous return in "This is Not Happening"/"Deadalive". Actor Terry O'Quinn, who appears in this episode as the Shadow Man, had appeared as different characters in the second season episode "Aubrey" and the 1998 feature film and appeared as Peter Watts in The X-Files sister show Millennium. The tagline for the episode is "They're Watching."