N}t}}\)Where,K = Rate CONSTANT which depends on the type of microorganism, the type of disinfectant and temperaturen = Constant for a particular microorganism and type of disinfectantC = Disinfectant concentration (weight/volume)Nt = Number of microorganism present at any time ‘t’CALCULATION:Given, t = 8 min, n = 1 and C = 0.1 g/m3∵ We know that, \({N_t} = {N_o}{e^{ - K{C^n}t}}\)Let 'x' be the number of micro organism present initially99% KILL of micro organism implies that at time 't' 1% of micro organism are still surviving∴ Micro organism surviving at time 't' = (1/100) x∴ \(\frac{1}{{100}}x = x.{e^{ - K \times 0.1 \times 8}}\)⇒ \( - K \times 0.8 = \ln \left( {\frac{1}{{100}}} \right)\)K = 5.76 /min